Color reaction between pyrogallol red-molybdenum(VI) complex and protein.
نویسندگان
چکیده
منابع مشابه
DIFFERENTIAL PULSE POLAROGRAPHIC DETERMINATION OF OSMIUM BY CATALYSIS OF PYROGALLOL-RED BROMATE REACTION
A highly sensitive differential pulse polarographic (DPP) method has been developed for the determination of trace amounts of osmium, based on the catalytic effect of osmium (VIII) on the oxidation of pyrogallol red (PGR) by sodium bromate at pH 7.00 and 25.0 0.1 ? C. The reaction rate was monitored by measuring the differential pulse cathodic current of the oxidation product of PGR and broma...
متن کاملUrinary protein as measured with a pyrogallol red-molybdate complex, manually and in a Hitachi 726 automated analyzer.
In this new method for determining urinary protein, the reaction is complete within 10 min at 37 degrees C. This method is applicable to automated as well as manual measurements. Protein concentration and absorbance at 600 nm are linearly related throughout a wide range of concentrations, 10 to 16 000 mg/L. However, the chromogenicity of the gamma-globulins in this method is 70% of that of albu...
متن کاملdifferential pulse polarographic determination of osmium by catalysis of pyrogallol-red bromate reaction
a highly sensitive differential pulse polarographic (dpp) method has been developed for the determination of trace amounts of osmium, based on the catalytic effect of osmium (viii) on the oxidation of pyrogallol red (pgr) by sodium bromate at ph 7.00 and 25.0 0.1 ? c. the reaction rate was monitored by measuring the differential pulse cathodic current of the oxidation product of pgr and bromate...
متن کاملTotal protein determination in urine: elimination of a differential response between the coomassie blue and pyrogallol red protein dye-binding assays.
BACKGROUND The total protein content of urine is a good index of renal function, but its determination is unreliable. Protein dye-binding assays are simple, but they characteristically lack a uniform response to different proteins. METHODS We investigated a differential response of the Sigma Microprotein Coomassie Brilliant Blue (CBB) and Pyrogallol Red-molybdate (PRM) protein dye-binding ass...
متن کاملKinetics of the Reaction of Pyrogallol Red, a Polyphenolic Dye, with Nitrous Acid: Role of NO and NO2.
In the present work we studied the reaction under gastric conditions of pyrogallol red (PGR), a polyphenolic dye, with nitrous acid (HONO). PGR has been used as a model polyphenol due to its strong UV-visible absorption and its high reactivity towards reactive species (radicals and non-radicals, RS). The reaction was followed by UV-visible spectroscopy and high performance liquid chromatography...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1983
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.32.12_e379